Correlate. Visualise. Analyse 

Relate is a correlative software package optimised to work with multimodal microscopy datasets, including Electron Microscopy (EM), Energy-dispersive X-ray spectroscopy (EDS), Electron Backscatter Diffraction (EBSD), Raman spectroscopy, Light microscopy and Atomic Force Microscopy (AFM). It provides the tools you need to correlate data from different microscopes, visualise multi-layered data in 2D and 3D, and conduct correlative analyses.

Analysing and correlating data from different modalities is a common challenge for users of multiple imaging systems. Often, correlating images and visualising the correlative data is a complicated process, including different software solutions and file conversions.

AFM Topography
Circuit board AFM topography overlayed with EDS image maps
AFM Topography + EDS
Circuit board AFM topography 3D display in Relate
3d view of correlated images from afm and eds displaying topographical and chemical information simultaneously

Relate can import datasets from different modalities and file formats, correlate them and generate multi-layered images.​ With Relate, the visualisation of correlative microscopy is interactive, with different 2D and 3D rendering options within a single document.

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  • Import data from AZtec using the HDF (H5oina) file format​
  • Import data from different microscope modalities (SEM, TEM, AFM, Raman, Light microscopy)​
  • Correlate multimodal data using intuitive image overlays and image matching tools​
  • Produce combined multimodal datasets​


  •  2D display of multi-layered data​
  •  3D visualisation of image files properties (e.g. topography combined with AFM material properties, EM images with correlative LM, Raman, EDS & EBSD map overlays) ​
  •  Customisation of colour palettes, data overlays, image rendering options and document display​
  •  Export images and animations​


  •  Generate multilayer profiles (cross section) views of 3D topographical displays
  •  Measure and quantify data across multiple layers​
  •  Analyse areas via data thresholding using metadata information including amount of X-ray counts, phase maps, height, or other material properties using measurement tools​
  •  Select an extensive range of measurement parameters​
  •  Create analysis templates for high throughput reporting​
  •  Export analytical data to text or CSV files​
Features Relate Software Stereoscopy Option Image Analysis Option
Import HDF5 data stacks, IBW (Asylum Research AFM) files and common image formats (e.g. TIFF, PNG, JPEG) N/A N/A
Correlation of data stacks and images​ N/A N/A
Multiple layer 2D and 3D data visualisation ​ N/A N/A
Display optimisation (e.g. editable palettes, lighting, rescaling, rotation, rendering options)​ N/A N/A
Image rendering, 3D animation and video export  N/A N/A
Profile extraction from 2D and 3D data N/A N/A
Quantification of threshold-based ROI selection N/A N/A
Select and export quantitative correlated data from ROI  N/A N/A
Document workflows and construct template N/A N/A
3D reconstruction from stereoscopic SEM images
3D Reconstruction from single EM and 4-quadrent BSE images
 EM image colourisation​
Import custom scripting from MATLAB
Texture analysis
Image segmentation by threshold or morphological filters 
Statistical analysis of morphology
System Requirements
Operating systems​Windows 10, 64 bit (recent version still supported by Microsoft)​
Windows 11, 64 bit
Recommended processorFor standard use: multi-core processor​
For intensive use: multiple multi-core processors
DiskMinimum: 1 GB of HDD free space​
RAM​Minimum: 4 GB​
Recommended for standard use: 8 GB​
Recommended for intensive use: 32 GB​
Graphics​Minimum: OpenGL 2.0 or Direct X 9.0c support​
Recommended: Dedicated GPU 1 GB
Screen resolution​Minimum: 1280×768​
Recommended: Full HD
Other1 free USB port if software use is protected by a “physical” key (USB dongle)​
Software-based security keys (code delivered via email) are also available (subject to conditions); the license is attached to one PC only

A: Yes, please follow the Free Trial link in Relate main page to download from the eStore.

A: Relate is compatible with hundreds of file formats. Here are a list of compatible file formats.

A: Relate is a data analysis software. The correlation workflow is done post-acquisition.

A: No. Relate allows you to export documents pages as PDF, RTF and PNG. Processed images can be exported with the most common image formats (e.g TIFF, PNG, BMP) and analytical data can be exported as text or CSV.

Your operative system must be Windows 11, 64 bit (or Windows 10, 64 bit).

Tutorial 1

How to create and customise Relate documents

Tutorial 3

How to correlate multimodal microscopy

Tutorial 2

How to load, display and resample your data

Tutorial 4

How to use the manual measurements operator

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