Nacre of pearls and mollusc shells is a hybrid nano composite of aragonite (CaCO3) platelets and biopolymers. The colorful luster
of nacre is due to the aragonite platelet thickness which is close to the wavelength of visible light. Further, the hybrid composite
nanostructure lends nacre extraordinary mechanical properties. The biopolymer component provides flexibility and tensile strength
while, the mineral component is essential for high elastic modulus, compressive and bending strength, as well as hardness and
abrasive resistance. The fracture toughness of nacre is orders of magnitude higher than that of pure CaCO3 (Mayer, 2005,
Barthelat, 2010). Due to the large electron penetration depth in nacre at the conventionally used 20 kV acceleration voltage in
SEM/EBSD investigations, a spatial resolution in the order of 1 micron has been reported. (Schmahl et al., 2009, Goetz et al., 2009). This is insufficient to resolve nacreous nanostructures. This application note illustrates how the resolution of EBSD data can be
significantly enhanced when low acceleration voltage is used.